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Blue native PAGE stained with Coomassie and zymography simultaneously. The HMW Native Marker ladder (GE Healthcare) values are given in kDa. The dark, purple stain results from the zymography, whereas the lighter, blue stain is caused by the Coomassie blue G-250 treatment

Journal: Applied Microbiology and Biotechnology

Article Title: Coupled reactions by coupled enzymes: alcohol to lactone cascade with alcohol dehydrogenase–cyclohexanone monooxygenase fusions

doi: 10.1007/s00253-017-8501-4

Figure Lengend Snippet: Blue native PAGE stained with Coomassie and zymography simultaneously. The HMW Native Marker ladder (GE Healthcare) values are given in kDa. The dark, purple stain results from the zymography, whereas the lighter, blue stain is caused by the Coomassie blue G-250 treatment

Article Snippet: Precast native PAGE gradient gels were ordered from Bio-Rad.

Techniques: Blue Native PAGE, Staining, Zymography, Marker